Biotechnology
GATE BT Analytical tools PYQs
Build your understanding with focused practice from past exam papers.
How to practice Analytical tools
Attempt a question, record the concept and assumptions, then check the available answer. Rework mistakes after revising the relevant concepts. Topic tags can overlap; review the question context.
Questions 1–10 of 20. Imported answers require independent review unless marked reviewed.
Question 14
Open questionBTMCQ1 markNeeds review
Cancer cells display a high level of heterogeneity in the expression of TP53 protein. Which of the following techniques can be directly used to determine the intra-tumoral heterogeneity of TP53 expression in a given tumor sample, without resorting to additional cell separation techniques?
Your answerChoose one option.
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Open questionBTMSQ2 marksNeeds review
Which of the following methods is/are used for identifying histone modifications?
Your answerSelect all correct options. You can choose more than one.
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You can attempt this question now. Sign in to view the answer key, explanations and discussion.Log in to check answerQuestion 60
Open questionBTNAT2 marksNeeds review
The percentage of light that would pass through a sample with an absorbance of 2 would be ______ %.
(Round off to the nearest integer)
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You can attempt this question now. Sign in to view the answer key, explanations and discussion.Log in to check answerQuestion 64
Open questionBTNAT2 marksNeeds review
An NMR spectrometer operating at proton resonance frequency (ν) of 1 GHz will have a magnetic field strength of ________ Tesla (T).
The gyromagnetic ratio for proton, γ = 2.675 × 10
T
s
(Round off to one decimal place)
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You can attempt this question now. Sign in to view the answer key, explanations and discussion.Log in to check answerQuestion 41
Open questionBTMCQ2 marksNeeds review
Match the item in Column I with the corresponding technique in Column II.
Column I:
P. Blue laser
Q. Tungsten filament
R. ¹⁵N labeled protein
S. Polyacrylamide
Column II:
Electron microscopy
Fluorescence activated cell sorting
Electrophoresis
Nuclear magnetic resonance spectroscopy
Your answerChoose one option.
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Open questionBTMCQ1 markNeeds review
In ELISA, which of the following enzymes are conjugated to antibodies for detection of the analyte?
P. Alkaline phosphatase
Q. Trypsinase
R. Horseradish peroxidase
S. Amylase
Your answerChoose one option.
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You can attempt this question now. Sign in to view the answer key, explanations and discussion.Log in to check answerQuestion 28
Open questionBTMCQ1 markNeeds review
Which one of the following methods CANNOT be used to determine the secondary structure content of a protein?
Your answerChoose one option.
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You can attempt this question now. Sign in to view the answer key, explanations and discussion.Log in to check answerQuestion 57
Open questionBTNAT2 marksNeeds review
A protein has three identical sites arranged at the vertices of an equilateral triangle. If one site is filled with a dye (donor), the measured quantum yield (
) is 0.5. Filling one site with a donor dye and a second site with an acceptor dye results in
of 0.25. The measured
of one site filled with donor and the other two sites filled with acceptor dye (rounded off to three decimal places) is __________.Log in to check the answer
You can attempt this question now. Sign in to view the answer key, explanations and discussion.Log in to check answerQuestion 45
Open questionBTMCQ2 marksNeeds review
Which of the following spectroscopic technique(s) can be used to identify all the functional group of an antibiotic contaminant in food?
P. Infrared
Q. Circular dichroism
R. Nuclear magnetic resonance
S. UV-Visible
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You can attempt this question now. Sign in to view the answer key, explanations and discussion.Log in to check answerQuestion 34
Open questionBTMCQ2 marksNeeds review
Tertiary structure of a protein consisting of α-helices and β-strands can be determined by
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