Biotechnology
GATE BT Analytical tools PYQs
Build your understanding with focused practice from past exam papers.
Questions 11–20 of 20. Imported answers require independent review unless marked reviewed.
Question 38
Open questionBTMSQ2 marksNeeds review
Which of the following nucleus/nuclei is/are NMR active?
Your answerSelect all correct options. You can choose more than one.
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Open questionBTMCQ1 markNeeds review
Which one of the following techniques can be used to compare the expression of a large number of genes in two biological samples in a single experiment?
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You can attempt this question now. Sign in to view the answer key, explanations and discussion.Log in to check answerQuestion 32
Open questionBTMCQ2 marksNeeds review
Group I lists spectroscopic methods and Group II lists biomolecular applications of these methods. Match the methods in Group I with the applications in Group II.
Group I
P. Infrared
Q. Circular Dichroism
R. Nuclear Magnetic Resonance
Group II
Identification of functional groups
Determination of secondary structure
Estimation of molecular weight
Determination of 3-D structure
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Open questionBTNAT2 marksNeeds review
A UV-visible spectrophotometer has a minimum detectable absorbance of 0.02. The minimum concentration of a protein sample that can be measured reliably in this instrument with a cuvette of 1 cm path length is ______ μM. The molar extinction coefficient of the protein is 10,000 L mol⁻¹ cm⁻¹.
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You can attempt this question now. Sign in to view the answer key, explanations and discussion.Log in to check answerQuestion 12
Open questionBTMCQ1 markNeeds review
Which of the following technique(s) can be used to study conformational changes in myoglobin?
P. Mass spectrometry
Q. Fluorescence spectroscopy
R. Circular dichroism spectroscopy
S. Light microscopy
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You can attempt this question now. Sign in to view the answer key, explanations and discussion.Log in to check answerQuestion 23
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The absorbance of a solution of tryptophan measured at 280 nm in a cuvette of 2.0 cm path length is 0.56 at pH 7. The molar extinction coefficient (ε) for tryptophan at 280 nm is 5600 M⁻¹cm⁻¹ at pH 7. The concentration of tryptophan (in μM) in the solution is ________
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You can attempt this question now. Sign in to view the answer key, explanations and discussion.Log in to check answerQuestion 5
Open questionBTNAT1 markNeeds review
In a thin layer chromatography experiment using a silica gel plate, a compound showed migration of 12.5 cm and the solvent front showed migration of 18 cm. The Rf value for the compound is ______.
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You can attempt this question now. Sign in to view the answer key, explanations and discussion.Log in to check answerQuestion 9
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Which one of the following techniques can be used to determine the structure of a 15 kDa globular protein at atomic resolution?
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You can attempt this question now. Sign in to view the answer key, explanations and discussion.Log in to check answerQuestion 12
Open questionBTMCQ1 markNeeds review
Enzyme-linked immunosorbent assay (ELISA) is used for
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You can attempt this question now. Sign in to view the answer key, explanations and discussion.Log in to check answerQuestion 30
Open questionBTMCQ2 marksNeeds review
The circular dichroism spectra of three proteins P, Q, and R are given below:
Choose the correct combination.
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