GATE BT Bioreactor engineering PYQs & Answers

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Questions 1–10 of 35. Imported answers require independent review unless marked reviewed.

Topic practice 35 questionsPage 1 of 4

If the rate at which E. coli divides is 0.5 h

, then its doubling time is ________ h.

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Under complete cell washout condition in a chemostat with sterile feed, which of the following statements is/are correct?

Your answerSelect all correct options. You can choose more than one.

Biomass concentration in the reactor is maximum

Substrate concentration in the exit stream is less than that in the inlet stream

Substrate concentration in the exit stream is equal to that in the inlet stream

Substrate concentration in the exit stream is zero

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Aspergillus niger is grown in a 10,000 L stirred batch bioreactor under aerated conditions to produce citric acid. At steady state oxygen transfer conditions, the specific oxygen uptake rate of the organism and the volumetric mass transfer coefficient are

and 60 min

, respectively.

If the oxygen solubility is

kg m

under the operating conditions, based only on oxygen dynamics, the maximum possible cell concentration is ______ kg m

(Answer in integer).

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A fed batch process is running at quasi-steady state with respect to substrate and biomass concentration. At 2 h, the culture volume is 500 L with a constant sterile inlet feed at 50 L h

of glucose. The culture kinetic parameters

and

are 0.2 h

and 0.1 g L

, respectively.

The substrate concentration in the reactor will be ______ g L

(rounded off to one decimal place).

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Consider scale-up of fungal fermentation from a 20 L model-type to 20,000 L prototype stirred tank reactor. The model-type and prototype have the same aspect ratio during scale-up. The impeller speed in the model-type is 500 rpm and the scale-up criterion is constant shear.

The impeller speed in the prototype reactor will be ______ rpm (Answer in integer).

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Temperature of a reaction with an activation energy value of 15 kcal.mol⁻¹ is increased from 300 K to 310 K. If the value of the ideal gas constant (R) is 1.9872 cal.mol⁻¹.K⁻¹, the ratio of the reaction rate constants (k₃₁₀/k₃₀₀) (rounded off to two decimal places) is __________.

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E. coli is cultivated in a chemostat operated at a dilution rate of 0.2 h⁻¹. The values of biomass yield due to oxygen consumption and the steady state biomass concentration are 0.2 g.g⁻¹ and 10 g.L⁻¹, respectively. The oxygen transfer rate (in g.L⁻¹.h⁻¹) is __________.

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The following schematic diagram shows a chemostat with cell recycle

where F₀ and Fᵣ are the volumetric flow rates (in L.h

) of feed and recycle streams, respectively. X₁, X₀ and X are the cell concentrations (in g.L

) in the reactor, recycle-stream and product-stream, respectively. If

and X₁ is 7.3 g.L

, the value of X (in g.L

, rounded off to one decimal place) is __________.

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A microorganism is grown in a batch culture using glucose as a carbon source. The apparent growth yield is 0.5

. The initial concentrations of biomass and substrate are 2 g L

and 200 g L

, respectively. Assuming that there is no endogenous metabolism, the maximum biomass concentration that can be achieved is ________________ g L

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The specific growth rate of a yeast having a doubling time of 0.693 h (rounded off to nearest integer) is ________________ h

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GATE BT Bioreactor engineering PYQs

How to practice Bioreactor engineering

Question 34

Question 53

Question 55

Question 60

Question 61

Question 47

Question 48

Question 53

Question 28

Question 33