GATE BT Bioreactor engineering PYQs & Answers — Page 2

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Questions 11–20 of 35. Imported answers require independent review unless marked reviewed.

Topic practice 35 questionsPage 2 of 4

Which of the following statements are CORRECT for an enzyme entrapped in a spherical particle?

Your answerSelect all correct options. You can choose more than one.

Effectiveness factor is ratio of the reaction rate with diffusion-limitation to the reaction rate without diffusion-limitation

Internal diffusion is rate-limiting at low values of Thiele modulus

Effectiveness factor increases with decrease in Thiele modulus

Internal diffusion-limitation can be reduced by decreasing the size of the particle

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The amount of biomass in a reactor at the end of the batch process is 50 g. Fed-batch operation is initiated by feeding the substrate solution at a constant rate of 1 L h

. The concentration of substrate in the feed is 50 g L

. The maximum biomass yield (

) is 0.4

. Assuming the system is at quasi-steady state, the maximum amount of biomass after 5 h of feeding is ____________ g.

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An enzyme catalyzes the conversion of substrate A into product B. The rate equation for this reaction is

Substrate A at an initial concentration of 10 mol L

enters an ideal mixed flow reactor (MFR) at a flow rate of 10 L min

. The volume of the MFR required for 50% conversion of substrate to product is __________ L.

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Consider the growth of S. cerevisiae under aerobic condition in a bioreactor and the specific growth rate of yeast is 0.5 h

. The overall reaction of the process is

The heat of combustion values for different compounds are tabulated below with the reference to CO

, H

O, O

, and N

at standard conditions.

Compound	Heat of combustion (kJ mol )
C H O	2802
NH	383
CH O N	560
C H O	1366

The specific rate of heat production (rounded off to nearest integer) is ____________ kJ mol

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A bacterial strain is grown in nutrient medium at 37 °C under aerobic conditions. The medium is inoculated with 10

cells from a seed culture. If the number of cells in the culture is 10

after 10 hours of growth, the doubling time of the strain (rounded off to nearest integer) is ____________ h.

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Which one of the following represents non-growth associated product formation kinetics in a bioprocess system?

and

denote viable cell and product concentrations, respectively.

Your answerChoose one option.

Diagram for Biotechnology question 26 (figure 2)

Diagram for Biotechnology question 26 (figure 3)

Diagram for Biotechnology question 26 (figure 4)

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In a chemostat with a dilution rate of 0.8 h⁻¹, the steady state biomass concentration and the specific product formation rate are 8 mol m⁻³ and 0.2 (mol product)(mol biomass)⁻¹ h⁻¹, respectively. The steady state product concentration in mol m⁻³ is _____.

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It is desired to scale-up a fermentation from 1 L to 1000 L vessel by maintaining a constant power-to-volume ratio. The small fermenter is operated at an agitator speed of 300 rotations per minute (rpm). If the value of scale up factor is 10, agitator speed in rpm (rounded off to the nearest integer) for the large fermenter is _____.

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The specific growth rate of a mold during exponential phase of its growth in a batch cultivation is 0.15 h⁻¹. If the cell concentration at 30 h is 33 g L⁻¹, the cell concentration in g L⁻¹ (rounded off to the nearest integer) at 24 h is _____.

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A batch cultivation of

follows zero order Monod’s growth kinetics. The cell growth is terminated when the residual dissolved oxygen concentration attains 10% of its saturation value and oxygen mass transfer coefficient (

) reaches its maximum value (

). The saturation value of dissolved oxygen concentration is

. If the maximum specific growth rate and yield coefficient (

) are

and

, respectively, then the final cell concentration in

(rounded off to two decimal places) at the end of the batch cultivation is ________.

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GATE BT Bioreactor engineering PYQs

Question 48

Question 58

Question 59

Question 62

Question 65

Question 26

Question 42

Question 45

Question 46

Question 51