GATE BT branch:bt PYQs & Answers — Page 50

Questions 491–500 of 650. Imported answers require independent review unless marked reviewed.

Topic practice 650 questionsPage 50 of 65

In general, which one of the following statements is NOT CORRECT?

Your answerChoose one option.

Hydrogen bonds result from electrostatic interactions

Hydrogen bonds contribute to the folding energy of proteins

Hydrogen bonds are weaker than van der Waals interactions

Hydrogen bonds are directional

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For site-directed mutagenesis, which one of the following restriction enzymes is used to digest methylated DNA?

Your answerChoose one option.

KpnI

DpnI

XhoI

MluI

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Match the organelles in Group I with their functions in Group II.

Group I P. Lysosome Q. Smooth ER R. Golgi apparatus S. Nucleolus

Group II

Digestion of foreign substances

Protein targeting

Lipid synthesis

Protein synthesis

rRNA synthesis

Your answerChoose one option.

P-1, Q-3, R-2, S-5

P-1, Q-4, R-5, S-3

P-2, Q-5, R-3, S-4

P-1, Q-3, R-4, S-5

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Which of the following statements are CORRECT when a protein sequence database is searched using the BLAST algorithm?

P. A larger E-value indicates higher sequence similarity Q. E-value < 10⁻¹⁰ indicates sequence homology R. A higher BLAST score indicates higher sequence similarity S. E-value > 10¹⁰ indicates sequence homology

Your answerChoose one option.

P, Q and R only

Q and R only

P, R and S only

P and S only

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Which one of the following is coded by the ABO blood group locus in the human genome?

Your answerChoose one option.

Acyl transferase

Galactosyltransferase

Transposase

β-Galactosidase

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Which of the following factors affect the fidelity of DNA polymerase in polymerase chain reaction?

P. Mg²⁺ concentration Q. pH R. Annealing temperature

Your answerChoose one option.

P and Q only

P and R only

Q and R only

P, Q and R

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Group I lists spectroscopic methods and Group II lists biomolecular applications of these methods. Match the methods in Group I with the applications in Group II.

Group I P. Infrared Q. Circular Dichroism R. Nuclear Magnetic Resonance

Group II

Identification of functional groups

Determination of secondary structure

Estimation of molecular weight

Determination of 3-D structure

Your answerChoose one option.

P-4, Q-3, R-1

P-2, Q-1, R-3

P-1, Q-2, R-4

P-3, Q-2, R-4

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The hexapeptide P has an isoelectric point (pI) of 6.9. Hexapeptide Q is a variant of P that contains valine instead of glutamate at position 3. The two peptides are analyzed by polyacrylamide gel electrophoresis at pH 8.0. Which one of the following statements is CORRECT?

Your answerChoose one option.

P will migrate faster than Q towards the anode

P will migrate faster than Q towards the cathode

Both P and Q will migrate together

Q will migrate faster than P towards the anode

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Antibody-producing hybridoma cells are generated by the fusion of a

Your answerChoose one option.

T cell with a myeloma cell

B cell with a myeloma cell

macrophage with a myeloma cell

T cell and a B cell

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Which of the following statements are CORRECT about the function of fetal bovine serum in animal cell culture?

P. It stimulates cell growth Q. It enhances cell attachment R. It provides hormones and minerals S. It maintains pH at 7.4

Your answerChoose one option.

P and Q only

P and S only

P, Q and R only

P, Q, R and S

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GATE BT branch:bt PYQs

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