Biotechnology
GATE BT Engineering principles applied to biological systems PYQs
Build your understanding with focused practice from past exam papers.
Questions 11–20 of 21. Imported answers require independent review unless marked reviewed.
Question 57
Open questionBTNAT2 marksNeeds review
Figure below depicts simplified metabolic and transport reactions taking place in the production of B from A in a cell. The subscript ‘i’ refers to intracellular metabolites.
is the
reaction flux in
. Under pseudo-steady-state condition, the following reaction fluxes are available.
,
and
.The transport flux of B,
, is ______________
. 
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Open questionBTNAT2 marksNeeds review
A feed stream (
) containing components
and
is processed in a system comprising of separation unit and a mixer as shown below in the schematic diagram. The mole fraction of the components
and
are
and
, respectively. If
, the degrees of freedom of the system is ________. 
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You can attempt this question now. Sign in to view the answer key, explanations and discussion.Log in to check answerQuestion 55
Open questionBTNAT2 marksNeeds review
A bacterium produces acetic acid from ethanol as per the following reaction
The thermodynamic maximum yield of acetic acid from ethanol in
(rounded off to two decimal places) is ________.Log in to check the answer
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Open questionBTNAT1 markNeeds review
The elemental composition of dry biomass of a yeast species is CH₁.₆O₀.₄N₀.₂S₀.₀₀₂₄P₀.₀₁₇. The contribution of carbon to the dry biomass is ____________% (round off to 2 decimal places).
[Given: atomic weights of H, C, N, O, P and S are 1, 12, 14, 16, 31 and 32, respectively]
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Open questionBTMCQ1 markNeeds review
Which one of the following need NOT be conserved in a biochemical reaction?
Your answerChoose one option.
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The degree of reduction for acetic acid (C₂H₄O₂) is __________.
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The mass of 1 kmol of oxygen molecules is ______ g (rounded off to the nearest integer).
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Open questionBTNAT2 marksNeeds review
Yeast biomass (C₆H₁₀O₃N) grown on glucose is described by the stoichiometric equation given below:
C₆H₁₂O₆ + 0.48 NH₃ + 3 O₂ → 0.48 C₆H₁₀O₃N + 3.12 CO₂ + 4.32 H₂O
The amount of glucose needed for the production of 50 g L⁻¹ of yeast biomass in a batch reactor with a working volume of 1,00,000 L is ______ kg (rounded off to the nearest integer).
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Open questionBTNAT2 marksNeeds review
Yeast converts glucose to ethanol and carbon dioxide by glycolysis as per the following reaction:
Assuming complete conversion, the amount of ethanol produced (in g) from 200 g of glucose is (up to two decimal places)
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Open questionBTNAT2 marksNeeds review
If the chemical composition of proteins in an organism is CH₁.₅O₀.₃N₀.₃S₀.₀₀₄, the mass percentage of carbon in the proteins is ________.
Given data: Atomic weights (Da) of C = 12, H = 1, O = 16, N = 14, and S = 32.
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