Biotechnology
GATE BT exam:gate PYQs
Build your understanding with focused practice from past exam papers.
Questions 31–40 of 650. Imported answers require independent review unless marked reviewed.
Question 31
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The mean of the following series is _____. (rounded off to three decimal places)
0.01, 0.02, 0.03, ⋯ ⋯ ⋯, 1
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You are characterizing a new enzyme isolated and purified in the laboratory. If the maximum velocity of the enzyme is 1800 μmoles L⁻¹ min⁻¹ and the total concentration of the enzyme in the reaction mixture is 1.5 μM, then the turnover number of the enzyme is _____ s⁻¹. (answer in integer)
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To determine the viable cell count of a bacterial culture, you have plated 50 μL of a 100-fold diluted sample of the culture on a nutrient agar plate and obtained 20 colonies after overnight incubation. The viable cell count of the culture is _____ CFU mL⁻¹. (answer in integer)
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Given below is the Gibbs free energy change (ΔG) in kilo Joules per electron equivalent (kJ e⁻eq⁻¹) at pH 7.0, of organic and inorganic half reactions.
Acetate synthesis:
ΔG=27.4 kJ e⁻eq⁻¹Reduction reaction:
ΔG = -78.72 kJ e⁻eq⁻¹The free energy change of acetate oxidation to CO₂, H₂O and HCO₃⁻ is _____ kJ e⁻eq⁻¹. (rounded off to two decimal places)
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You have purified an enzyme using a series of chromatographic methods. It was observed that a 10 μg mL⁻¹ of this purified enzyme converted 10 mM substrate per hour at 25°C and pH 7. Its specific activity is _____ IU μg⁻¹. (rounded off to three decimal places)
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Open questionBTMCQ2 marksNeeds review
In the open-loop process shown in the figure, the input
, the transfer function
and the output
are given in the Laplace domain in terms of the Laplace variable s. For this process, which of the following is true?(where
,
,
, are the magnitude of the input, the characteristic time and the gain for the process, respectively)
Your answerChoose one option.
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Match the immunological terms in Column I with their function/description in the Column II
Column I: P. J gene; Q. Cyclosporin; R. Epitope; S. Hapten
Column II: 1. Suppresses immune system by blocking calcineurin; 2. Antibody binding site on a large molecule; 3. Small foreign molecule which elicits an immune response only when combined with a carrier molecule; 4. Contributes to antibody diversity
Your answerChoose one option.
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Match the genetic disorders in Column I to the corresponding underlying cause in Column II
Column I: P. Klinefelter Syndrome; Q. Turner syndrome; R. Bloom syndrome; S. Xeroderma pigmentosum
Column II: 1. X-chromosome insufficiency; 2. DNA helicase mutations; 3. Nucleotide excision repair defects; 4. Extra X chromosome(s) in a male
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Match the biomolecules in Column I with their function given in Column II
Column I: P. Lectins; Q. P-glycoprotein; R. Digitoxigenin; S. Mannose 6-phosphate
Column II: 1. Acts as ATP dependent pump to efflux out small molecules; 2. Targets lysosomal enzymes to their destination; 3. Specific carbohydrate binding proteins; 4. Inhibits Na⁺-K⁺ pump
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By elemental analysis of a biomass sample, it was found that the cells in the given sample are composed of (by weight) C=50%, H=8%, O=20%, N=10% and ash content=12%. Which of the following is the correct empirical formula of the ash-free cells, normalized with respect to Nitrogen?
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