Biotechnology
GATE BT format:mcq PYQs
Build your understanding with focused practice from past exam papers.
Questions 371–380 of 389. Imported answers require independent review unless marked reviewed.
Question 41
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The genome is diploid at the end of which phases of a human mitotic cell cycle?
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A recombinant protein is to be expressed under the control of the lac promoter and operator in a strain of E. coli having the genotype lac⁺ crp⁺. Even in the absence of inducer IPTG, low levels of expression of the recombinant protein are seen (leak expression). Which one of the following should be done to minimize such leak expression?
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Shown below is a plasmid vector (P) and an insert (Q). The insert was cloned into the BamHI site of the vector. The recombinant plasmid was isolated and digested with BamHI or XhoI. The results from the digestion experiments are shown in (R).

Which one of the following explains the digestion results shown in (R)?
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Which one of the following CANNOT be a recognition sequence for a Type II restriction enzyme?
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A pedigree of an inheritable disease is shown below.

This inheritable disease is
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Growth of a microbe in a test tube is modeled as
, where, X is the biomass, r is the growth rate, and K is the carrying capacity of the environment (
;
). If the value of starting biomass is
, which one of the following graphs qualitatively represents the growth dynamics?Your answerChoose one option.
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The interaction energy E between two spherical particles is plotted as a function of the distance (r) between them. When
, where
is a constant, the net force between the spherical particles is repulsive. When
, they attract via van der Waals attraction. Which one of the following plots correctly represents the interaction energy between the above two particles?Your answerChoose one option.
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EcoRI restriction sites on a 10kb DNA fragment are shown below.

Upon partial digestion, what are the lengths (in kb) of all the possible DNA fragments obtained?
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Which of the following conditions promote the development of human autoimmune disorders?
P. Inability to eliminate self-reactive lymphocytes
Q. Generation of auto-antibodies
R. Ability to eliminate self-reactive T-cells
S. Induction of regulatory T-cells in the thymus
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She has a sharp tongue and it can occasionally turn ________.
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