Questions 371–380 of 389. Imported answers require independent review unless marked reviewed.

Topic practice 389 questionsPage 38 of 39

Question 41

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The genome is diploid at the end of which phases of a human mitotic cell cycle?
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Question 42

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A recombinant protein is to be expressed under the control of the lac promoter and operator in a strain of E. coli having the genotype lac⁺ crp⁺. Even in the absence of inducer IPTG, low levels of expression of the recombinant protein are seen (leak expression). Which one of the following should be done to minimize such leak expression?
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Question 43

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Shown below is a plasmid vector (P) and an insert (Q). The insert was cloned into the BamHI site of the vector. The recombinant plasmid was isolated and digested with BamHI or XhoI. The results from the digestion experiments are shown in (R).
Diagram for Biotechnology question 43

Which one of the following explains the digestion results shown in (R)?
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Question 44

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Which one of the following CANNOT be a recognition sequence for a Type II restriction enzyme?
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Question 45

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A pedigree of an inheritable disease is shown below.
Diagram for Biotechnology question 45

This inheritable disease is
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Question 48

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Growth of a microbe in a test tube is modeled as

, where, X is the biomass, r is the growth rate, and K is the carrying capacity of the environment (

;

). If the value of starting biomass is

, which one of the following graphs qualitatively represents the growth dynamics?
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Question 50

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The interaction energy E between two spherical particles is plotted as a function of the distance (r) between them. When

, where

is a constant, the net force between the spherical particles is repulsive. When

, they attract via van der Waals attraction. Which one of the following plots correctly represents the interaction energy between the above two particles?
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Question 51

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EcoRI restriction sites on a 10kb DNA fragment are shown below.
Diagram for Biotechnology question 51

Upon partial digestion, what are the lengths (in kb) of all the possible DNA fragments obtained?
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Question 55

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Which of the following conditions promote the development of human autoimmune disorders?
P. Inability to eliminate self-reactive lymphocytes Q. Generation of auto-antibodies R. Ability to eliminate self-reactive T-cells S. Induction of regulatory T-cells in the thymus
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Question 56

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She has a sharp tongue and it can occasionally turn ________.
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