GATE BT format:nat PYQs & Answers — Page 2

Questions 11–20 of 201. Imported answers require independent review unless marked reviewed.

Topic practice 201 questionsPage 2 of 21

A CSTR with a volume of 100 m

is operated in cell recycle mode. At a volumetric flow rate of 10 m

day

and effluent biomass of 20 mg L

, the steady state biomass concentration is 200 mg L

. The mean cell retention time in the reactor is ____ days. (answer in integer)

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The electron equivalent per liter (e

eq L

) of acetate ion solution of concentration 10 g L

is ______ e

eq L

. (rounded off to one decimal place)

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From the following plot of

versus

and if y(2) = 5, the value of y(3) is ______. (rounded off to one decimal place)

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A batch sterilizer is being operated at 121 °C for sterilizing a medium containing microbial cells. Assume that the thermal inactivation of cells is a first order process with a death rate constant of 0.69 min

at 121 °C. If the initial concentration of microbes in the medium is 10

cells m

, the time taken to reduce the microbial load to a final concentration of 10 cells m

is ________ min. (rounded off to the nearest integer)

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A substrate is consumed in a zero order reaction such that its concentration falls from 42 g L

to 14 g L

in 4 hours. The total time taken for complete utilization of substrate will be ________ hours. (answer in integer)

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A microbe that follows Monod growth kinetics on a limiting substrate (maximum specific growth rate of 0.5 h

and Monod constant of 0.1 mg L

) is cultivated in a continuous reactor for microbial growth (chemostat with sterile feed). Given that the chemostat volume is 5 L and inlet concentration of limiting substrate is 10 g L

, the minimum inlet feed flow rate for chemostat washout is ______ L h

. (rounded off to one decimal place)

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It is desired to cultivate 150 mg (dry weight) microbial cells with an empirical formula of C

N. If the phosphorus requirement for the cells is 20% of the nitrogen requirement (on a weight basis), the minimum amount of phosphorus required to be added to the cultivation medium is _______ mg. (rounded off to one decimal place)

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In the process of generating a clonal mammalian cell line, a single cell was seeded in a well of a cell culture plate. After the first 48 hours, one of the progeny cells underwent apoptosis due to a new mutation. If the doubling time of the cells is 24 hours and no more cell death occurs, the total number of cells after a total of 7 days from seeding will be _____. (answer in integer)

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A disease-associated allele shows X-linked recessive inheritance. If the mother is a carrier of the disease, the father is genetically normal and the child born is a son, then the probability that he is born with the disease is _____. (rounded off to one decimal place)

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A packed bed bioreactor with length 1 m and inside diameter 10 cm has liquid flowing at an interstitial velocity of 1 cm s⁻¹. Given a volumetric flow rate of 0.025 L s⁻¹, the void fraction of the packed bed is _____. (rounded off to two decimal places)

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GATE BT format:nat PYQs

Question 56

Question 57

Question 58

Question 59

Question 60

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Question 62

Question 63

Question 64

Question 65