GATE BT Molecular tools PYQs & Answers

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Attempt a question, record the concept and assumptions, then check the available answer. Rework mistakes after revising the relevant concepts. Topic tags can overlap; review the question context.

Questions 1–10 of 19. Imported answers require independent review unless marked reviewed.

Topic practice 19 questionsPage 1 of 2

Which component of the CRISPR/Cas9 gene editing system is NOT of natural origin?

Your answerChoose one option.

Cas9 protein

CRISPR repeats

PAM sequence

sgRNA

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Which one of the following is the basic principle of Sanger’s DNA sequencing method?

Your answerChoose one option.

Chain termination by incorporation of dideoxynucleotides

Chain elongation by incorporation of dideoxynucleotides

Release of inorganic pyrophosphate

Chain cleavage by modification of deoxynucleotides

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Match the chemical (Column I) with its use (Column II).

Column I Column II P. Diethylpyrocarbonate 1. Chelation of magnesium ion during DNA purification Q. Cesium chloride 2. Prevention of RNA degradation in aqueous environment R. Ethidium bromide 3. Separation of DNA by density gradient centrifugation S. Ethylenediaminetetraacetic acid 4. Staining of RNA in agarose gel

Your answerChoose one option.

P-4; Q-1; R-3; S-2

P-4; Q-3; R-2; S-1

P-2; Q-1; R-4; S-3

P-2; Q-3; R-4; S-1

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Which of the following features help(s) in distinguishing alleles using restriction fragment length polymorphism (RFLP)?

Your answerSelect all correct options. You can choose more than one.

Differences in the number of recognition sites for a given restriction enzyme

Differences in the ability of alleles to undergo recombination

Differences in the ability of alleles to undergo segregation

Differences in the number of tandem repeats

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Which of the following methods are used for detection of DNA and RNA, respectively?

Your answerChoose one option.

Southern and Northern blotting

Southern and Western blotting

Northern and Southern blotting

Northern and Western blotting

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A dNTP master-mix is prepared by combining 40 μL of each 20 mM dNTP stock (dATP, dCTP, dGTP and dTTP). 4 μL of this dNTP master-mix is added to a PCR mix and the final volume is adjusted to 50 μL. The concentration (in μM) of total dNTPs in the PCR mix is ____________.

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Among individuals in a human population, minor variations exist in nucleotide sequences of chromosomes. These variations can lead to gain or loss of sites for specific restriction enzymes. Which of the following technique is used to identify such variations?

Your answerChoose one option.

Polymerase dependent fragment insertion

Real-time polymerase chain reaction

Restriction fragment length polymorphism

Reverse transcriptase polymerase chain reaction

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Which one of the following techniques/tools is NOT used for inserting a foreign gene into a cell?

Your answerChoose one option.

DNA microarray

Electroporation

Gene gun

Microinjection

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CRISPR-Cas system is associated with

Your answerChoose one option.

adaptive immunity in eukaryotes

adaptive immunity in prokaryotes

innate immunity in eukaryotes

innate immunity in prokaryotes

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The enzyme which adds phosphate group to the free 5′ terminus of a DNA sequence is

Your answerChoose one option.

adenosine kinase

alkaline phosphatase

polynucleotide kinase

terminal deoxynucleotidyl transferase

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GATE BT Molecular tools PYQs

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