GATE BT topic:animals PYQs & Answers

Attempt a question, record the concept and assumptions, then check the available answer. Rework mistakes after revising the relevant concepts. Topic tags can overlap; review the question context.

Questions 1–10 of 21. Imported answers require independent review unless marked reviewed.

Topic practice 21 questionsPage 1 of 3

For monoclonal antibody production, hypoxanthine ____________.

Your answerChoose one option.

allows DNA synthesis in the absence of de novo dTMP synthesis

allows purine synthesis in cells containing HGPRT

induces cell fusion

prevents de novo nucleotide synthesis

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In the process of generating a clonal mammalian cell line, a single cell was seeded in a well of a cell culture plate. After the first 48 hours, one of the progeny cells underwent apoptosis due to a new mutation. If the doubling time of the cells is 24 hours and no more cell death occurs, the total number of cells after a total of 7 days from seeding will be _____. (answer in integer)

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Which one of the following hosts is used in mammalian cell culture for the production of glycosylated recombinant therapeutic proteins?

Your answerChoose one option.

Pichia pastoris

Sf9 cells

Escherichia coli

Chinese hamster ovary cells

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A cultured skin fibroblast cell of a goat ‘P’ was fused with an enucleated ovum of a goat ‘Q’. The resultant activated early embryo was then transplanted into a pseudopregnant (surrogate) female goat ‘R’ of the same strain as ‘Q’. On completion of gestation, a female goat ‘S’ was born. With the exception of mitochondrial DNA, ‘S’ is a clone of ________.

Your answerChoose one option.

Only P

Only Q

Only R

Both P and R

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Which one of the following is an insect cell line?

Your answerChoose one option.

HEK 293

Sf9

DH5α

CHO

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Match the item (Column I) with its corresponding use (Column II).

Column I Column II P. Glutamine 1. Detachment of adherent cells Q. Trypsin 2. Selection of transfected mammalian cell lines R. Hypoxanthine 3. Source of carbon and nitrogen in animal cell culture media S. Neomycin 4. A component of medium for selection of hybrid in monoclonal antibody production

Your answerChoose one option.

P-3; Q-1; R-4; S-2

P-1; Q-2; R-4; S-3

P-3; Q-1; R-2; S-4

P-2; Q-3; R-1; S-4

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In hybridoma technology, which one of the following enzymes is absent in the myeloma cells that are used for monoclonal antibody production?

Your answerChoose one option.

Hypoxanthine-guanine phosphoribosyltransferase

Alanine aminotransferase

Triose phosphate isomerase

Glycosyltransferase

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Which of the following statement(s) is(are) TRUE about induced pluripotent stem cells?

Your answerSelect all correct options. You can choose more than one.

They can self-renew

They require specific signals to maintain their stemness

They cannot be genetically manipulated

They can form organoids in vitro

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Match the media component used in mammalian cell culture (Column I) with its respective role (Column II).

Column I P. Hydrocortisone Q. Fibronectin R. Epidermal growth factor S. Riboflavin

Column II

Mitogen

Vitamin

Hormone

Cell attachment

Your answerChoose one option.

P-3, Q-4, R-1, S-2

P-3, Q-4, R-2, S-1

P-4, Q-3, R-1, S-2

P-4, Q-3, R-2, S-1

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Which one of the following statements is INCORRECT about hybridoma production?

Your answerChoose one option.

Hybridoma cells can use hypoxanthine and thymidine

DNA synthesis in myeloma cells is blocked by aminopterin

Hybridoma cells are made to produce polyclonal antibodies

Polyethylene glycol is used to fuse myeloma cells to B-cells

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GATE BT topic:animals PYQs

How to practice topic:animals

Question 18

Question 63

Question 20

Question 20

Question 22

Question 39

Question 23

Question 41

Question 37

Question 7