Biotechnology
GATE BT topic:biochemistry PYQs
Build your understanding with focused practice from past exam papers.
Questions 21–30 of 52. Imported answers require independent review unless marked reviewed.
Question 21
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Intracellular proteins are targeted for proteolytic degradation in proteasomes upon conjugation with
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The number of different possible ways of forming five intramolecular disulfide bonds with ten cysteine residues of a protein is __________.
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An enzyme (E) catalyzes the biochemical reaction A → B with k₍cat₎ equal to 500 s
. If the initial reaction velocity (V₀) is 10 μM.s
at the total enzyme concentration [Eₜ] of 30 nM and substrate concentration [A] of 40 μM, the value of Kₘ (in μM) is __________.Log in to check the answer
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Which of the following statements about reversible enzyme inhibitors are CORRECT?
P. Uncompetitive inhibitors bind only to the enzyme-substrate complex
Q. Non-competitive inhibitors bind only at a different site from the substrate
R. Competitive inhibitors bind to the same site as the substrate
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Terpenoids are made of __________ units
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Which of the following are CORRECT about protein structure?
P. Secondary structure is formed by a repeating pattern of interactions among the polypeptide backbone atoms
Q. Tertiary structure is the three-dimensional arrangement of the polypeptide backbone atoms only
R. Quaternary structure refers to an assemble of multiple polypeptide subunits
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Which of the following is(are) COMMON feature(s) for both aerobic and anaerobic bacterial cultures?
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Which of the following plot(s) is(are) CORRECT for an enzyme that obeys Michaelis-Menten kinetics, assuming
?
is the concentration of the substrate,
is the Michaelis constant, and
is the initial reaction velocity.Your answerSelect all correct options. You can choose more than one.
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You can attempt this question now. Sign in to view the answer key, explanations and discussion.Log in to check answerQuestion 14
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A protein without its prosthetic group is known as
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The enzyme
-amylase used in starch hydrolysis has an affinity constant (
) value of
. To achieve one-fourth of the maximum rate of hydrolysis, the required starch concentration in
(rounded off to two decimal places) is ________.Log in to check the answer
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