Questions 241–250 of 650. Imported answers require independent review unless marked reviewed.

Topic practice 650 questionsPage 25 of 65

Question 46

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The following reaction represents biomass synthesis from hexadecane
C₁₆H₃₄ + 12.5O₂ + 2.13NH₃ → 10.6CH₁.₆₆O₀.₂₇N₀.₂₇ + 5.37CO₂ + 11.4H₂O
where CH₁.₆₆O₀.₂₇N₀.₂₇ represents the biomass. The value of respiratory quotient (rounded off to two decimal places) is __________.

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Question 47

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Temperature of a reaction with an activation energy value of 15 kcal.mol⁻¹ is increased from 300 K to 310 K. If the value of the ideal gas constant (R) is 1.9872 cal.mol⁻¹.K⁻¹, the ratio of the reaction rate constants (k₃₁₀/k₃₀₀) (rounded off to two decimal places) is __________.

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Question 48

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E. coli is cultivated in a chemostat operated at a dilution rate of 0.2 h⁻¹. The values of biomass yield due to oxygen consumption and the steady state biomass concentration are 0.2 g.g⁻¹ and 10 g.L⁻¹, respectively. The oxygen transfer rate (in g.L⁻¹.h⁻¹) is __________.

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Question 49

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Aqueous two-phase extraction is used to recover α-amylase from a solution. A polypropylene glycol-dextran mixture is added and the solution separates into upper and lower phases. The partition coefficient is 4.0 and the ratio of the upper to lower phase volume is 5.0. The enzyme recovery or yield (in percentage, rounded off to the nearest integer) is __________.

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Question 50

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E. coli cultivated at 298 K uptakes an uncharged compound (A) by passive diffusion. The intracellular and extracellular concentrations of A are 0.001 M and 0.1 M, respectively. If the value of the ideal gas constant R is 1.9872 cal.mol

.K

, the free-energy change (in kcal.mol

) for this passive diffusion of A (rounded off to two decimal places) is __________.

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Question 51

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If there are three unrooted trees for four protein sequences, the number of rooted trees for the same number of sequences is __________.

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Question 52

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The number of different possible ways of forming five intramolecular disulfide bonds with ten cysteine residues of a protein is __________.

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Question 53

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The following schematic diagram shows a chemostat with cell recycle
Diagram for Biotechnology question 53

where F₀ and Fᵣ are the volumetric flow rates (in L.h

) of feed and recycle streams, respectively. X₁, X₀ and X are the cell concentrations (in g.L

) in the reactor, recycle-stream and product-stream, respectively. If

,

and X₁ is 7.3 g.L

, the value of X (in g.L

, rounded off to one decimal place) is __________.

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Question 54

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An enzyme (E) catalyzes the biochemical reaction A → B with k₍cat₎ equal to 500 s

. If the initial reaction velocity (V₀) is 10 μM.s

at the total enzyme concentration [Eₜ] of 30 nM and substrate concentration [A] of 40 μM, the value of Kₘ (in μM) is __________.

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Question 55

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DNA sample collected from an unidentified bacterial species (Y) contains 13% of adenine. The G+C content (in percentage) of Y is __________.

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