GATE BT Upstream and Downstream Processing PYQs & Answers

Questions 1–10 of 17. Imported answers require independent review unless marked reviewed.

Which one of the following proteins would elute third in gel filtration chromatography from a mixture of four proteins given below?

Your answerChoose one option.

Insulin (6 kDa)

Myoglobin (17 kDa)

Thrombin (37 kDa)

Albumin (66 kDa)

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A batch sterilizer is being operated at 121 °C for sterilizing a medium containing microbial cells. Assume that the thermal inactivation of cells is a first order process with a death rate constant of 0.69 min

at 121 °C. If the initial concentration of microbes in the medium is 10

cells m

, the time taken to reduce the microbial load to a final concentration of 10 cells m

is ________ min. (rounded off to the nearest integer)

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In adsorption chromatography, the adsorption of uncharged solute molecules onto a silica-based stationary phase is by ________.

Your answerChoose one option.

covalent bonds

electrostatic interactions

ionic bonds

van der Waals forces

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The decimal reduction time of a microbe during sterilization at 120 °C with a first order thermal death rate constant of 1 min

will be ________ min (rounded off to 1 decimal place).

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Aqueous two-phase extraction is used to recover α-amylase from a solution. A polypropylene glycol-dextran mixture is added and the solution separates into upper and lower phases. The partition coefficient is 4.0 and the ratio of the upper to lower phase volume is 5.0. The enzyme recovery or yield (in percentage, rounded off to the nearest integer) is __________.

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Match the media component (Column I) with its role (Column II).

Column I	Column II
P. Sucrose	1. Anti-foam agent
Q. Zinc chloride	2. Nitrogen source
R. Ammonium sulphate	3. Carbon source
S. Silicone oil	4. Trace element

Your answerChoose one option.

P-1, Q-2, R-3, S-4

P-2, Q-1, R-3, S-4

P-3, Q-2, R-4, S-1

P-3, Q-4, R-2, S-1

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Match the stationary phase (Column I) with its corresponding chromatography technique (Column II).

Column I P. Protein A Q. Sephadex R. Phenylsepharose S. Diethylaminoethyl cellulose

Column II

Size exclusion chromatography

Ion-exchange chromatography

Affinity chromatography

Hydrophobic interaction chromatography

Your answerChoose one option.

P-1, Q-4, R-2, S-3

P-3, Q-1, R-4, S-2

P-3, Q-4, R-2, S-1

P-4, Q-1, R-3, S-2

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A pilot sterilization was carried out in a vessel containing 100 m

medium with an initial spore concentration of 10

spores/ml. The accepted level of contamination after sterilization is 1 spore in the entire vessel. The specific death rate constant for the spore is 2 min

at 121 °C. Assuming no death takes place during the heating and cooling cycles, the holding time at 121 °C (rounded off to nearest integer) is ____________ min.

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Match separation methods in Group I with associated properties in Group II.

Group I P. Centrifugation Q. Dialysis R. Solvent extraction S. Ultrafiltration

Group II

Density

Diffusivity

Size

Solubility

Your answerChoose one option.

P-4, Q-2, R-1, S-3

P-3, Q-1, R-2, S-4

P-1, Q-3, R-2, S-4

P-1, Q-2, R-4, S-3

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Two monomeric His-tagged proteins of identical molecular weight are present in a solution. pIs of these two proteins are 5.6 and 6.8. Which one of the following techniques can be used to separate them?

Your answerChoose one option.

Denaturing polyacrylamide gel electrophoresis

Size-exclusion chromatography

Ion-exchange chromatography

Nickel affinity chromatography

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GATE BT Upstream and Downstream Processing PYQs

How to practice Upstream and Downstream Processing

Question 19

Question 59

Question 11

Question 35

Question 49

Question 14

Question 40

Question 63

Question 28

Question 8