Biotechnology
GATE BT year:2019 PYQs
Build your understanding with focused practice from past exam papers.
Questions 41–50 of 65. Imported answers require independent review unless marked reviewed.
Question 31
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Which of the following factors affect the fidelity of DNA polymerase in polymerase chain reaction?
P. Mg²⁺ concentration
Q. pH
R. Annealing temperature
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Group I lists spectroscopic methods and Group II lists biomolecular applications of these methods. Match the methods in Group I with the applications in Group II.
Group I
P. Infrared
Q. Circular Dichroism
R. Nuclear Magnetic Resonance
Group II
Identification of functional groups
Determination of secondary structure
Estimation of molecular weight
Determination of 3-D structure
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The hexapeptide P has an isoelectric point (pI) of 6.9. Hexapeptide Q is a variant of P that contains valine instead of glutamate at position 3. The two peptides are analyzed by polyacrylamide gel electrophoresis at pH 8.0. Which one of the following statements is CORRECT?
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Antibody-producing hybridoma cells are generated by the fusion of a
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Which of the following statements are CORRECT about the function of fetal bovine serum in animal cell culture?
P. It stimulates cell growth
Q. It enhances cell attachment
R. It provides hormones and minerals
S. It maintains pH at 7.4
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Which one of the following covalent linkages exists between 7-Methyl guanosine (m⁷G) and mRNAs?
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Which one of the following amino acid residues will destabilize an α-helix when inserted in the middle of the helix?
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What is the solution of the differential equation
, with the initial condition, at
,
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The Laplace transform of the function
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Which of the following factors can influence the lag phase of a microbial culture in a batch fermenter?
P. Inoculum size
Q. Inoculum age
R. Medium composition
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