Biotechnology
GATE BT year:2021 PYQs
Build your understanding with focused practice from past exam papers.
Questions 61–65 of 65. Imported answers require independent review unless marked reviewed.
Question 51
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A batch cultivation of
follows zero order Monod’s growth kinetics. The cell growth is terminated when the residual dissolved oxygen concentration attains 10% of its saturation value and oxygen mass transfer coefficient (
) reaches its maximum value (
). The saturation value of dissolved oxygen concentration is
. If the maximum specific growth rate and yield coefficient (
) are
and
, respectively, then the final cell concentration in
(rounded off to two decimal places) at the end of the batch cultivation is ________.Log in to check the answer
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Milk flowing through a stainless steel inner tube (40 mm inner diameter) of double tube-type heater is to be heated from
to
by saturated steam condensing at
on the outer surface of the inner tube. Total heat transferred (
) is
and the overall heat transfer coefficient is
. The total length of the heating tube in
(rounded off to one decimal place) is ________.Log in to check the answer
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A DNA solution of
concentration gives an absorbance of 1.0 at 260 nm. An aliquot of
from a
purified plasmid solution is diluted with distilled water to a total volume of
. The diluted plasmid solution gives an absorbance of 0.50 at 260 nm. The concentration of the purified plasmid in
(rounded off to two decimal places) is ________.Log in to check the answer
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The possible number of SalI restriction sites in a 9 kb double-stranded DNA, with all four bases occurring in equal proportion (rounded off to the nearest integer) is ________.
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A bacterium produces acetic acid from ethanol as per the following reaction
The thermodynamic maximum yield of acetic acid from ethanol in
(rounded off to two decimal places) is ________.Log in to check the answer
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