GATE XL branch:xl PYQs & Answers — Page 29

Questions 281–290 of 1235. Imported answers require independent review unless marked reviewed.

Which one of the following is the correct match between the molecular properties listed in Column I and the corresponding biochemical separation methods in Column II?

Your answerChoose one option.

P-i; Q-ii; R-v; S-iii

P-iii; Q-iv; R-ii; S-i

P-iii; Q-iv; R-i; S-ii

P-v; Q-iv; R-iii; S-ii

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Which one or more of the following statements is/are correct regarding the electromotive force generated by electron transfer chain?

Your answerSelect all correct options. You can choose more than one.

It is used for the synthesis of ATP.

It is not used for active transport process.

It includes a pH gradient component.

It does not include an electrical potential gradient component.

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Which one or more of the following statements is/are correct regarding the transport and retention of proteins in different cell organelles?

Your answerSelect all correct options. You can choose more than one.

Mannose 6-phosphate residues are involved in targeting proteins to lysosomes.

Transport of proteins into the mitochondrial compartment is aided by positively charged amino acid residues at the N-terminus and internal hydrophobic segments.

The retention of protein in the ER lumen requires the KDE sequence motif at the C-terminus.

Nuclear proteins are transported in an unfolded conformation and the nuclear localization signal sequence is subsequently cleaved by peptidases in the nucleoplasm.

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Which one or more of the following statements correctly describe(s) fluorescence spectroscopy?

Your answerSelect all correct options. You can choose more than one.

The emission maxima (λₘₐₓ) is independent of the excitation wavelength.

The emission maxima (λₘₐₓ) depends on the concentration of a quencher.

The emission maxima (λₘₐₓ) varies with solvent polarity.

The emission maxima (λₘₐₓ) varies with temperature.

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Which one or more of the following statements is/are correct in the processing of pre-mRNA in eukaryotes?

Your answerSelect all correct options. You can choose more than one.

3′ → 5′ exonuclease activity is involved in the conversion of pre-mRNA to mRNA.

5′-cap and addition of 3′-poly A tail precedes splicing.

Splicing of pre-mRNA occurs via transesterification reaction.

Alternative splicing can yield different mRNA products from the same pre-mRNA.

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Which one or more of the following statements correctly describe(s) the changes upon the addition of puromycin during eukaryotic translation?

Your answerSelect all correct options. You can choose more than one.

Puromycin resembles aminoacyl end of the charged tRNA.

Puromycin occupies the A site of the translating ribosomes.

Puromycin occupies the P site of the translating ribosomes.

Puromycin occupies the E site of the translating ribosomes.

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Factor H, a complement regulator protein in plasma, binds C3b and

Your answerSelect all correct options. You can choose more than one.

competes with factor B to displace Bb from convertase.

initiates the catabolism of C3b into inactive products.

then binds to C3bBb convertase.

acts as a cofactor for factor I.

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In Michaelis-Menten’s equation, if [S] = 15

, then the ratio

is __________. (Round off to three decimal places)

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A 520 base-pair long plasmid with 10 negative supercoils would have a linking number of __________, considering 10.5 base pairs per turn for B DNA. (Answer in integer)

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The spectrum of a protein obtained using electrospray ionization mass spectrometry (ESI-MS) is shown below. Two peaks, one at m/z = 296.0 and the other at m/z = 3552.5, are marked. The mass of the protein associated with the m/z = 296.0 peak is __________ Da. (Round off to two decimal places)

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GATE XL branch:xl PYQs

Question 37

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