GATE XL format:nat PYQs & Answers — Page 13 — Exam Papers

Questions 121–130 of 244. Imported answers require independent review unless marked reviewed.

Topic practice 244 questionsPage 13 of 25

One mole of a circular bacterial plasmid was digested with a high-fidelity restriction enzyme. The plasmid has five restriction sites for the enzyme used. The number of moles of fragments released upon cleavage at all sites is ________.

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The genome of a bacterium encodes for 10 different surface antigens, whose expression can be turned ‘ON’ or ‘OFF’ randomly and independently. The number of possible antigenic combinations is __________.

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Suppose the mRNAs in a newly discovered bacteria are composed of only two distinct nucleotides (as opposed to four found in all known organisms). Considering that the organism has no nucleotide modification systems, the number of nucleotides required per codon to encode at least 20 distinct amino acids will be __________.

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The decimal reduction time (D) for reducing 10¹² spores of Clostridium botulinum to 1 spore at 111°C will be ______ min (in integer). The D value is 0.2 min at 121°C. The increase in temperature required to change D to 1/10ᵗʰ of its initial value (Z value) is 10°C.

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The generation time of E. coli is 30 minutes. For an exponentially growing culture, the initial number of bacteria required to reach a number of 10⁹ in 2 hours is __________×10⁷ (round off to two decimal places).

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A man, whose mother and father had blood groups A and O respectively, marries a woman with blood group AB. If the man has blood group A, then the number of different blood groups possible among their children will be ________ (in integer).

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A population of snakes in an isolated island is in Hardy-Weinberg equilibrium for a gene with only two alleles (A and a). If the allelic frequency of A is 0.6, then the genetic frequency of Aa is ________ (round off to 2 decimal places).

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In the structure of a polypeptide, one α-helix (3.6₃ helix) contains 32 intra-chain hydrogen bonds. The number of turns in the helix will be ________ (in integer).

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An enzyme catalyzes the conversion of 30 μM of a substrate to product at reaction velocity of 9.0 μM s⁻¹. When [Eₜ] = 30 nM and Kₘ = 10 μM, Kcat / Kₘ of enzyme will be n × 10⁷ M⁻¹ s⁻¹. The value of n is ________ (in integer).

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A cross is made between two animals of genotypes AaBb × AaBb, where loci A and B assort independently. The progeny of this dihybrid cross was then allowed to self-cross. The proportion of the progeny that showed segregation for loci A (i.e., produce A- and aa progeny) in % will be ________ (in integer).

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