GATE XL Plant Breeding, Genetic Modification, Genome Editing PYQs & Answers

Questions 1–10 of 18. Imported answers require independent review unless marked reviewed.

Correctly match the following molecules/methods in Group-I with their major uses in Group-II.

Group-I: (P) CRISPR-Cas9 (Q) DNA Microarray (R) Vir genes (S) RAPD

Group-II: (1) Global gene expression analysis (2) Molecular marker (3) Targeted gene editing (4) Agrobacterium-mediated plant transformation

Your answerChoose one option.

P–1; Q–3; R–2; S–4

P–3; Q–1; R–4; S–2

P–3; Q–1; R–2; S–4

P–1; Q–3; R–4; S–2

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Which of the following molecular approaches can be used to generate complete knock-out of a target gene in plants?

Your answerSelect all correct options. You can choose more than one.

Homologous recombination

CRISPR-Cas9

Antisense RNA technique

Activation tagging

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Correctly match the enzymes used as selectable markers (Group I) and the chemicals used for their selection (Group II).

Group I: (P) Neomycin phosphotransferase; (Q) Phosphinothricin acetyltransferase; (R) Dihydrofolate reductase; (S) 5-Enolpyruvyl shikimate 3-phosphate synthase. Group II: (1) Bialaphos; (2) Kanamycin; (3) Glyphosate; (4) Methotrexate

Your answerChoose one option.

P-2; Q-1; R-4; S-3

P-1; Q-2; R-3; S-4

P-2; Q-4; R-1; S-3

P-3; Q-4; R-1; S-2

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Which of the following selectable marker genes is/are used for herbicide tolerance during genetic transformation of plants?

Your answerSelect all correct options. You can choose more than one.

hpt

bar

nptII

pmi

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With reference to Agrobacterium tumefaciens mediated plant transformation, match the virulence factors in Group 1 with their protein types in Group 2.

Group 1: (P) VirG; (Q) VirA; (R) VirE; (S) VirC.

Group 2: (i) Kinase; (ii) Helicase; (iii) Transcriptional activator; (iv) Single strand binding protein

Your answerChoose one option.

P–i, Q–ii, R–iv, S–iii

P–iii, Q–i, R–ii, S–iv

P–ii, Q–iv, R–i, S–iii

P–iii, Q–i, R–iv, S–ii

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A drought tolerant rice genotype was found to be associated with a missense mutation in the gene A. Which one or more of the following experiments is/are appropriate to validate whether the mutation in A is the causal factor for drought tolerance?

Your answerSelect all correct options. You can choose more than one.

Introduce the same mutation in a drought sensitive rice genotype and test if it becomes drought tolerant.

Delete the wild-type A in drought sensitive plant and test if it becomes drought tolerant.

Determine the stability of the protein encoded by the wild-type and the mutant forms of A.

Repair the mutation in the drought tolerant rice genotype and test if it becomes drought sensitive.

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Match Group-I (selection agent) and Group-II (gene) in CORRECT combination.

Group-I: P. Kanamycin; Q. Hygromycin; R. Phosphinothricin; S. Mannose.

Group-II: I. pmi; II. bar; III. nptII; IV. ptxD; V. dhfr; VI. hpt

Your answerChoose one option.

P-III, Q-VI, R-II, S-I

P-IV, Q-II, R-II, S-I

P-I, Q-VI, R-III, S-II

P-II, Q-I, R-V, S-VI

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Which of the following enzyme(s), when overexpressed, would result in rice grains with increased β-carotene content?

Your answerSelect all correct options. You can choose more than one.

Phytoene synthase

Carotene desaturase

β-glucuronidase

Enolpyruvishikimate-3-phosphate synthase (EPSPS)

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Match the genetically modified crop in GROUP I with the corresponding genetic element in GROUP II.

GROUP I P. Tomato with delayed fruit ripening Q. Herbicide-resistant soybean R. Insect-resistant cotton S. Soybean with modified oil content

GROUP II

EPS synthase

Δ12-Desaturase

Polygalacturonase

Bt-Cry protein

Your answerChoose one option.

P-3, Q-1, R-4, S-2

P-1, Q-3, R-2, S-4

P-2, Q-1, R-4, S-3

P-3, Q-2, R-4, S-1

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Which of the following option(s) is/are CORRECT in the context of hybrid plant generation using Barnase/Barstar-based male sterile lines?

Your answerSelect all correct options. You can choose more than one.

Banase inhibits Barstar.

Barstar inhibits Banase.

Banase and Barstar are used to generate the male sterile line and the restorer line, respectively.

Banase and Barstar are used to generate the restorer line and the male sterile line, respectively.

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GATE XL Plant Breeding, Genetic Modification, Genome Editing PYQs

How to practice Plant Breeding, Genetic Modification, Genome Editing

Question 49

Question 53

Question 61

Question 52

Question 57

Question 61

Question 58

Question 9

Question 15

Question 18