GATE XL Plant Breeding, Genetic Modification, Genome Editing PYQs & Answers — Page 2

Questions 11–18 of 18. Imported answers require independent review unless marked reviewed.

Which of the following chemicals is used for doubling of chromosome numbers during production of ‘doubled haploids’ in crop plants?

Your answerChoose one option.

Hygromycin

Kanamycin

Colchicine

Glufosinate

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In relation to Agrobacterium mediated genetic engineering in plants, match the following in CORRECT combination

Gene name: P. virA; Q. virB; R. virD1; S. virG

Function: i. Acetosyringone receptor; ii. Conjug tube formation; iii. Topoisomerase; iv. Inducer of all vir operons; v. Octopine synthesis

Your answerChoose one option.

P-iv, Q-iii, R-ii, S-v

P-ii, Q-i, R-iii, S-v

P-i, Q-ii, R-iii, S-iv

P-iii, Q-i, R-ii, S-iv

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Selection markers and the corresponding genes used in plant genetic engineering are given below

Choose the CORRECT combination

Your answerChoose one option.

P-ii, Q-i, R-iv, S-iii

P-iv, Q-ii, R-i, S-iii

P-iv, Q-i, R-ii, S-iii

P-iii, Q-iv, R-ii, S-i

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The plant cells are considered totipotent. The phenomenon of a mature cell reverting to the meristematic state and forming undifferentiated callus tissue is called

Your answerChoose one option.

redifferentiation

dedifferentiation

organogenesis

recalcitrancy

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Cybirds may arise through the

Your answerChoose one option.

fusion of a normal protoplast with another nucleated protoplast of different origin

fusion between a normal protoplast and a protoplast containing viable nucleus

elimination of one of the nuclei from heterokaryon formed from two protoplasts of different origin

fusion of a normal cell with another nucleated cell

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Which one of the following methods is INCORRECT with respect to haploid plant production? It can be produced

Your answerChoose one option.

from an unfertilized egg cell

from nucellar tissue

from isolated pollen culture

by distant hybridization, followed by selective elimination of chromosomes of one of the parents

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You are asked to design a genetic construct for high-level expression of a gene encoding the therapeutic protein 18 (TP18) via plastid transformation. Select the CORRECT set of genetic elements for this construct.

Your answerChoose one option.

Actin1 promoter → TP18 coding sequence → Actin1 transcription terminator

Ubiquitin1 promoter → TP18 coding sequence → Ubiquitin1 transcription terminator

rbcS promoter → TP18 coding sequence → rbcS transcription terminator

rbcL promoter → TP18 coding sequence → rbcL transcription terminator

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Choose the CORRECT set of matches between group I and group II in relation to plant genetic transformation methods.

GROUP I P. Helium Q. Acetosyringone R. Polyethylene glycol S. Agarose embedding

GROUP II

Agrobacterium tumefaciens

Microinjection

Particle bombardment

Protoplast

Your answerChoose one option.

P-4, Q-3, R-2, S-1

P-2, Q-1, R-4, S-3

P-3, Q-4, R-1, S-2

P-3, Q-1, R-4, S-2

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GATE XL Plant Breeding, Genetic Modification, Genome Editing PYQs

Question 9

Question 13

Question 19

Question 8

Question 14

Question 18

Question 12

Question 52