GATE XL topic:microbial-growth PYQs & Answers — Page 2

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topic:microbial-growth

Questions 11–20 of 25. Imported answers require independent review unless marked reviewed.

Topic practice 25 questionsPage 2 of 3

Under anaerobic fermentative growth conditions, one mole of glucose yields 22 grams of Streptococcus faecalis or 8.6 grams of Zymomonas mobilis. The molar growth yield (YATP) for (i) S. faecalis and (ii) Z. mobilis will be

Your answerChoose one option.

(i) 11 and (ii) 4.3

(i) 22 and (ii) 4.3

(i) 22 and (ii) 8.6

(i) 11 and (ii) 8.6

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The generation time of E. coli is 30 minutes. For an exponentially growing culture, the initial number of bacteria required to reach a number of 10⁹ in 2 hours is __________×10⁷ (round off to two decimal places).

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A 250 μl of bacteriophage stock containing

phages/ml is added to 500 μl of E. coli culture containing

cells/ml. The multiplicity of infection is _____.

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A bacterial culture containing

live cells was exposed to a newly developed sterilizing agent. After 30 minutes of exposure, 3 live cells remained in culture. The decimal reduction time (in minutes) for the new agent is _____.

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A bacterial culture has a generation time of 34 minutes. The time taken (in minutes, rounded off to two decimal places) for the OD₅₀ of this exponentially growing culture to increase from 0.25 to 0.85 is _____.

Assume that OD₅₀ has a linear relationship with the cell density.

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A 100 μl aliquot (

dilution) of the bacterial culture plated on the nutrient agar gave 4 colonies. The bacterial stock concentration (in million cells/ml, rounded off to one decimal place) is _____.

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A continuous bacterial culture carried out in a chemostat is set to a flow rate of 40 ml/hr. The culture volume is equivalent to that of a cubical container having 10 cm sides. The dilution rate (in hr⁻¹, rounded off to two decimal places) of this system is _____.

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Secondary metabolites such as penicillin from mold are produced during the __________.

Your answerChoose one option.

lag phase

idiophase

log phase

decline phase

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A bacterial culture contains 500 organisms / mL in the exponential growth phase at 8 am in the morning. If you consider a generation time of 20 minutes, the total number of organisms / mL (round off to the nearest integer) after three hours (i.e. at 11 am in the morning) will be

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Consider a spherical bacterial cell with diameter 2 μm and a eukaryotic cell of spherical shape with a diameter of 20 μm. The surface area to volume (in μm⁻¹) ratio of the bacterial cell (round off to 1 decimal place) is ______ times more than that of the eukaryotic cell.

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GATE XL topic:microbial-growth PYQs

Question 11

Question 20

Question 10

Question 17

Question 18

Question 19

Question 20

Question 6

Question 10

Question 19