Biotechnology
2018 · Paper 1
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Complete GATE BT 2018 Paper 1 overview, original pages and practice · Timed previous-paper practice
Questions 64–65 of 65. Imported answers require review unless explicitly marked reviewed.
Question 54
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The product of complete digestion of the plasmid shown below with EcoRI and HaeII was purified and used as a template in a reaction containing Klenow fragment of DNA polymerase, dNTPs and [α-³²P]-dATP in a suitable reaction buffer. The product thus obtained was purified and subjected to gel electrophoresis followed by autoradiography.
The number of bands that will appear on the X-ray film is
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A rod shaped bacterium has a length of 2
m, diameter of 1
m and density the same as that of water. If proteins constitute 15% of the cell mass and the average protein has a mass of 50 kDa, the number of proteins in the cell is ________
(1 Da = 1.6 × 10⁻²⁴ g)Log in to check the answer
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