GATE BT 2023 Paper 1 — page 18

Questions 52–54 of 65. Imported answers require review unless explicitly marked reviewed.

The number of different possible ways of forming five intramolecular disulfide bonds with ten cysteine residues of a protein is __________.

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The following schematic diagram shows a chemostat with cell recycle

where F₀ and Fᵣ are the volumetric flow rates (in L.h

) of feed and recycle streams, respectively. X₁, X₀ and X are the cell concentrations (in g.L

) in the reactor, recycle-stream and product-stream, respectively. If

and X₁ is 7.3 g.L

, the value of X (in g.L

, rounded off to one decimal place) is __________.

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An enzyme (E) catalyzes the biochemical reaction A → B with k₍cat₎ equal to 500 s

. If the initial reaction velocity (V₀) is 10 μM.s

at the total enzyme concentration [Eₜ] of 30 nM and substrate concentration [A] of 40 μM, the value of Kₘ (in μM) is __________.

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2023 · Paper 1