GATE XL 2019 Paper 1 — page 15

Questions 43–45 of 125. Imported answers require review unless explicitly marked reviewed.

The C-terminal carboxyl group and the N-terminal amino group in amino acids have a dissociation constant (pKₐ) of 2.2 and 9.2, respectively. The pKₐ of side chain carboxyl group in glutamic acid is 4.2 and side chain amino group in lysine is 10.2. The difference in isoelectric point (pI) of lysine and glutamic acid (rounded off to two decimal places) is _____.

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X different sized DNA fragments can be observed upon incomplete EcoRI digestion of identical DNA molecules with two EcoRI sites as shown below. The maximum value of X is _____.

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An uncharged protein (P) has an Asp in position 23 with a molecular weight of 6501 Da, as determined by mass spectrometry. The uncharged mutant of this protein (P′) contains a single amino acid substitution with Asn at position 23. The molecular weight of P′, as determined by mass spectrometry (rounded off to one decimal place) is ____Da.

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2019 · Paper 1

Question 18

Question 19

Question 20