Question 18

XL-QNAT2 marksNeeds review
A PCR in a 100 μL reaction volume, containing two primers at a concentration of 0.2 μM each, is set up to amplify a 250 base pair DNA fragment. Consider the average molecular weight of one base pair as 660 Da. If the primers are fully consumed by the end of the reaction, the amount of the final PCR product formed is __________ μg (rounded off to one decimal place).

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2020XL-Q

GATE XL 2020 · Paper 1

Life Sciences · Original paper archive

Section
Section XL-Q
Question type
Numerical answer (NAT)
Marks
2 marks

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