GATE BT topic:life-sciences PYQs & Answers — Page 59

Questions 581–590 of 650. Imported answers require independent review unless marked reviewed.

Consider the following alignment of two DNA sequences:

Assuming an affine gap scoring scheme of an identity matrix for substitution, a gap initiation penalty of 1 and a gap extension penalty of 0.1, the score of the alignment is (up to one decimal place)

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First order deactivation rate constants for soluble and immobilized amyloglucosidase enzyme are 0.03 min⁻¹ and 0.005 min⁻¹, respectively. The ratio of half-life of the immobilized enzyme to that of the soluble enzyme is (rounded off to the nearest integer)

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Consider a simple uni-substrate enzyme that follows Michaelis-Menten kinetics. When the enzyme catalyzed reaction was carried out in the presence of 10 nM concentration of an inhibitor, there was no change in the maximal velocity. However, the slope of the Lineweaver-Burk plot increased 3-fold. The dissociation constant for the enzyme-inhibitor complex (in nM) is

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The product of complete digestion of the plasmid shown below with EcoRI and HaeII was purified and used as a template in a reaction containing Klenow fragment of DNA polymerase, dNTPs and [α-³²P]-dATP in a suitable reaction buffer. The product thus obtained was purified and subjected to gel electrophoresis followed by autoradiography.

The number of bands that will appear on the X-ray film is

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A rod shaped bacterium has a length of 2

m, diameter of 1

m and density the same as that of water. If proteins constitute 15% of the cell mass and the average protein has a mass of 50 kDa, the number of proteins in the cell is ________ (1 Da = 1.6 × 10⁻²⁴ g)

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An enzyme catalyzes a reaction by

Your answerChoose one option.

decreasing the energy of the substrate.

decreasing the activation energy of the reaction.

decreasing product stability.

increasing the activation barrier of the reaction.

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Natural proteins are composed primarily of 20 α-amino acids. Which one of the following statements is true for any of these amino acids in a solution of pH 1.5?

Your answerChoose one option.

Only the amino group is ionized.

Only the carboxylic acid group is ionized.

Both amino and carboxylic acid groups are ionized.

Both amino and carboxylic acid groups are neutral.

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Which one of the following organisms is an indicator of fecal contamination?

Your answerChoose one option.

Escherichia coli

Streptococcus lactis

Bacillus subtilis

Lactobacillus acidophilus

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The surface area (in m²) of the largest sphere that can fit into a hollow cube with edges of length 1 meter is _____.

Given data: π = 3.14

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In a thin layer chromatography experiment using a silica gel plate, a compound showed migration of 12.5 cm and the solvent front showed migration of 18 cm. The Rf value for the compound is ______.

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GATE BT topic:life-sciences PYQs

Question 51

Question 52

Question 53

Question 54

Question 55

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Question 5