GATE BT topic:recombinant-dna-technology PYQs & Answers

Questions 1–10 of 10. Imported answers require independent review unless marked reviewed.

Which of the following vector(s) is(are) used to clone a DNA fragment of size 220 kb?

Your answerSelect all correct options. You can choose more than one.

Bacterial artificial chromosome

Yeast artificial chromosome

Cosmids

pUC₁₉ plasmid

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The recognition sequences of four Type-II restriction enzymes (RE) are given below. The symbol (↓) indicates the cleavage site. Identify the RE that generates sticky ends.

Your answerChoose one option.

RE1 - 5′ G↓ATCC 3′

RE2 - 5′ TG↓CAG 3′

RE3 - 5′ CC↓GG 3′

RE4 - 5′ AG↓CT 3′

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A circular plasmid has three different but unique restriction sites for enzymes ‘a’, ‘b’ and ‘c’. When enzymes ‘a’ and ‘b’ are used together, two fragments of equal size are generated. Enzyme ‘c’ creates fragments of equal size only from one of the fragments generated by those cleaved by ‘a’ and ‘b’. The plasmid is treated with a mixture of ‘a’, ‘b’ and ‘c’ and analysed by agarose gel electrophoresis. The number of bands observed in the gel is __________.

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The possible number of SalI restriction sites in a 9 kb double-stranded DNA, with all four bases occurring in equal proportion (rounded off to the nearest integer) is ________.

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Which one of the following statements about Agrobacterium Ti plasmid is CORRECT?

Your answerChoose one option.

Vir genes are located within the T-DNA segment

Phytohormone biosynthesis genes are located outside the T-DNA segment

Opine catabolism genes are located within the T-DNA segment

Opine biosynthesis genes are located within the T-DNA segment

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The schematic of a plasmid with a gap in one of the strands is shown below:

Which of the following enzyme(s) is/are required to fill the gap and generate a covalently closed circular plasmid?

[P] DNA ligase

[Q] Alkaline phosphatase

[R] DNA polymerase

[S] Polynucleotide kinase

Your answerChoose one option.

P only

P, R and S only

P and R only

P, Q and R only

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Genomic DNA isolated from a bacterium was digested with a restriction enzyme that recognizes a 6-base pair (bp) sequence. Assuming random distribution of bases, the average length (in bp) of the fragments generated is ________

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The product of complete digestion of the plasmid shown below with EcoRI and HaeII was purified and used as a template in a reaction containing Klenow fragment of DNA polymerase, dNTPs and [α-³²P]-dATP in a suitable reaction buffer. The product thus obtained was purified and subjected to gel electrophoresis followed by autoradiography.

The number of bands that will appear on the X-ray film is

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Shown below is a plasmid vector (P) and an insert (Q). The insert was cloned into the BamHI site of the vector. The recombinant plasmid was isolated and digested with BamHI or XhoI. The results from the digestion experiments are shown in (R).

Which one of the following explains the digestion results shown in (R)?

Your answerChoose one option.

The insert did not ligate to the vector.

One copy of the insert ligated to the vector.

The insert ligated to the vector as two tandem copies.

The insert ligated to the vector as two copies but not in tandem.

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Which one of the following CANNOT be a recognition sequence for a Type II restriction enzyme?

Your answerChoose one option.

GAATTC

AGCT

GCGCCGC

ATGCCT

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GATE BT topic:recombinant-dna-technology PYQs

How to practice topic:recombinant-dna-technology

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