Question 64

BTNAT2 marksNeeds review
A circular plasmid has three different but unique restriction sites for enzymes ‘a’, ‘b’ and ‘c’. When enzymes ‘a’ and ‘b’ are used together, two fragments of equal size are generated. Enzyme ‘c’ creates fragments of equal size only from one of the fragments generated by those cleaved by ‘a’ and ‘b’. The plasmid is treated with a mixture of ‘a’, ‘b’ and ‘c’ and analysed by agarose gel electrophoresis. The number of bands observed in the gel is __________.

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2022BT

GATE BT 2022 · Paper 1

Biotechnology · Original paper archive

Section
Biotechnology
Question type
Numerical answer (NAT)
Marks
2 marks

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