Questions 771–780 of 1235. Imported answers require independent review unless marked reviewed.

Topic practice 1235 questionsPage 78 of 124

Question 11

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One mole of a native protein upon N-terminal analysis yielded one mole each of Asp and Val. Therefore, the protein in its native state exists as a
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Question 12

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The prosthetic groups/cofactors involved in both 1e

and 2e

transfer in the mitochondrial electron transport chain are
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Question 13

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Match the items in Group I with the most appropriate items in Group II and choose the correct option.
Group I: P. Integrin Q. Microglial cell R. TLR-7 S. Dendritic cell
Group II:
Phagocytosis in the neural tissue
Antigen processing by cross-presentation
Single stranded RNA recognition
Binding of cells to endothelium
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Question 14

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The correct combination of glycosidic linkages present in glycogen is
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Question 15

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Polypeptides are either biosynthesized on the ribosomes using an mRNA template or chemically synthesized by the Merrifield’s solid phase method. The correct directions of peptide synthesis are
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Question 16

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A solution absorbs 20% of the incident light in a cuvette of path length 1.0 cm. The amount of light transmitted by the same solution in a cuvette of 3.0 cm path length is ________% (rounded off to one decimal place).

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Question 17

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The second pKa of phosphoric acid is 6.8. The ratio of Na₂HPO₄ to NaH₂PO₄ required to obtain a buffer of pH 7.0 is ________ (rounded off to two decimal places).

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Question 18

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A PCR in a 100 μL reaction volume, containing two primers at a concentration of 0.2 μM each, is set up to amplify a 250 base pair DNA fragment. Consider the average molecular weight of one base pair as 660 Da. If the primers are fully consumed by the end of the reaction, the amount of the final PCR product formed is __________ μg (rounded off to one decimal place).

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Question 19

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An enzyme obeying Michaelis-Menten kinetics shows a reaction velocity (v) of 10 μmol/min when the substrate concentration [S] equals its Kₘ. The maximal velocity Vₘₐₓ for this enzyme is ______ μmol/min (correct to integer number).
(Kₘ is Michaelis-Menten constant)

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Question 20

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The enzyme glucose isomerase catalyses the inter-conversion of glucose and fructose as shown.
Glucose ⇌ Fructose
The ΔG′₀ for this reaction is zero kcal/mol. After adding glucose isomerase to a 0.12 M glucose solution and allowing the reaction to attain equilibrium, the final concentration of fructose in the reaction mixture will be ______ mM.

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