GATE XL 2020 Paper 1 — page 15

Questions 43–45 of 125. Imported answers require review unless explicitly marked reviewed.

A PCR in a 100 μL reaction volume, containing two primers at a concentration of 0.2 μM each, is set up to amplify a 250 base pair DNA fragment. Consider the average molecular weight of one base pair as 660 Da. If the primers are fully consumed by the end of the reaction, the amount of the final PCR product formed is __________ μg (rounded off to one decimal place).

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An enzyme obeying Michaelis-Menten kinetics shows a reaction velocity (v) of 10 μmol/min when the substrate concentration [S] equals its Kₘ. The maximal velocity Vₘₐₓ for this enzyme is ______ μmol/min (correct to integer number).

(Kₘ is Michaelis-Menten constant)

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The enzyme glucose isomerase catalyses the inter-conversion of glucose and fructose as shown.

Glucose ⇌ Fructose

The ΔG′₀ for this reaction is zero kcal/mol. After adding glucose isomerase to a 0.12 M glucose solution and allowing the reaction to attain equilibrium, the final concentration of fructose in the reaction mixture will be ______ mM.

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2020 · Paper 1

Question 18

Question 19

Question 20