GATE BT 2020 BT Q27: Molecular tools

The DNA sequence shown below is to be amplified by PCR:

5′ GCTAAGATCGAATTTC…..TTGGCAATAGTAGCGC 3′ 3′ CGATTCTAGCTTAAAG…..AACCGTTATCATCGCG 5′

Which one of the following pair of primers can be used for this amplification?

Your answerChoose one option.

5′ GGAATTCAGATCTAG 3′ and 5′ TGGCAATAGTAGCGC 3′

5′ GCTAAGATCGAATTTC 3′ and 5′ CGCTATTACCGTTA 3′

5′ GGAATTCAGATCTAG 3′ and 5′ CGCTATTACCGTTA 3′

5′ GCTAAGATCGAATTTC 3′ and 5′ TGGCAATAGTAGCGC 3′

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